Primer

Part:BBa_K1441015

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase Ecor1 F primer

This primer is specific to the Bio-bricks prefix preceding the DNA Ligase insert(BBa_K525710). This primer was designed to remove the stop codon present in the standardized iGEM prefixes. This allows for proper insertion and expression of the insert into pGAPz vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None